Journal: Scientific reports

Article Title: Protective effects of a new generation of probiotic Bacteroides fragilis against colitis in vivo and in vitro

doi: 10.1038/s41598-023-42481-8

Figure Lengend Snippet: Figure 5. B. fragilis inhibited TNF-α-induced generation of inflammation-associated cytokines. Culture supernatant was collected to value the concentrations of cytokines (a) IL-6; (b) IL-8; (c) IL-10; (d) IL-1β by Enzyme-linked immunosorbent assay. Expression of TLR2, TLR4, MYD88 were valued by western blot (e) and RT-qPCR (f–h) from molecular and protein level. ***p < 0.001, **p < 0.01, *p < 0.05 ns not significant. All data show mean ± SEM. The original western blot image was visible in Supplementary Material 2.

Article Snippet: Culture supernatant was collected from 6-well plates and the concentrations of cell cytokines (IL-6; IL-8; IL-10; IL-1β) were valued using enzyme-linked immunosorbent assay (ELISA) kits (Proteintech, Wuhan, China) and normalized to cell protein concentrations.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Quantitative RT-PCR

Journal: Scientific reports

Article Title: Protective effects of a new generation of probiotic Bacteroides fragilis against colitis in vivo and in vitro

doi: 10.1038/s41598-023-42481-8

Figure Lengend Snippet: Figure 6. B. fragilis culture supernatant attenuates TNF-α-induced cell death and inflammatory response. (a) Cell of survival rate of hcoEPIC cells. Culture supernatant was collected to value the concentrations of cytokines (b) IL-6; (c) IL-8; (d) IL-10; (e) IL-1β by Enzyme-linked immunosorbent assay. ***p < 0.001, **p < 0.01, *p < 0.05 ns not significant. All data show mean ± SEM. BFS, B. fragilis supernatant.

Article Snippet: Culture supernatant was collected from 6-well plates and the concentrations of cell cytokines (IL-6; IL-8; IL-10; IL-1β) were valued using enzyme-linked immunosorbent assay (ELISA) kits (Proteintech, Wuhan, China) and normalized to cell protein concentrations.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Spatial expression of an mRNA encoding Tie2-agonist in the capillary endothelium of the lung prevents pulmonary vascular leakage

doi: 10.1101/2022.10.12.511878

Figure Lengend Snippet: (A) Schematic diagram of the experimental protocol of the ex vivo perfused and ventilated mouse lung model. 6 h, 15 h after administration of mRNA-76-cLNP (1, 1.5 and 2 mg/kg) or control mRNA LUC -LNP (2 mg/kg) lungs were isolated and stimulated with PLY (1.4 μg/ml) for 1 minute. After 30 min, lung vascular permeability was assessed by quantifying respective concentrations of continuously infused human serum albumin (HSA) in the bronchoalveolar lavage fluid (BALF). (B) Graphic showing treatment with mRNA-76 to significantly decrease pneumolysin-induced hyperpermeability of mouse lungs as compared to treatment with control mRNA LUC -LNP 6 h (left) and 15 h (right) post mRNA-76-cLNP treatment as shown by HAS ELISA. Values are given as mean ( n = 10, **p<0.01 between indicated groups) concentration of human serum albumin (HSA) in bronchoalveolar lavage fluid (BALF). HSA concentration of individual mice are indicated as dots.

Article Snippet: Thirty minutes after PLY challenge, bronchoalveolar lavage (BAL) was performed (2 x 650 μl, 0.9 % saline buffer), and HSA concentration was measured in BAL fluid (BALF) via ELISA (Bethyl Laboratories, cat. #E88-129) in order to quantify the lung barrier failure ( ; ).

Techniques: Ex Vivo, Control, Isolation, Permeability, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: bioRxiv

Article Title: Spatial expression of an mRNA encoding Tie2-agonist in the capillary endothelium of the lung prevents pulmonary vascular leakage

doi: 10.1101/2022.10.12.511878

Figure Lengend Snippet: (A) Schematic drawing of the experimental protocol. Animals (n=10) were challenged intratracheally with 0.9% w/v saline or LPS (3 mg/kg). A fixed volume of 50 μL, equal to an approximate intratracheal dose volume of 2.5 mL/kg, based on a 20 g mouse, was applied intratracheally. 2h after LPS administration mRNA-76-cLNP (1.5 mg/kg IV) or mRNALUC-cLNP01 (1.5 mg/kg IV) was intravenously administered, and bronchoalveolar lavage fluid (BALF) was analysed after 24 h. (B) Effect of COMP-Ang1 expression on BALF total and differential cell counts and on wet lung weight in a murine model of endotoxin (LPS)-induced pulmonary inflammation. Data shown as mean ± standard error of the mean. *** p <0.001 when compared to saline challenged control group. # p <0.05, ## p <0.01, ### p <0.001 as compared to LPS challenged and vehicle treated group.

Article Snippet: Thirty minutes after PLY challenge, bronchoalveolar lavage (BAL) was performed (2 x 650 μl, 0.9 % saline buffer), and HSA concentration was measured in BAL fluid (BALF) via ELISA (Bethyl Laboratories, cat. #E88-129) in order to quantify the lung barrier failure ( ; ).

Techniques: Saline, Expressing, Control